Global and local selection acting on the pathogen Stenotrophomonas maltophilia in the human lung

[ { "id": "3607531", "title": "Rapid methicillin resistance diversification in Staphylococcus epidermidis colonizing human neonates", "abstract": "Early in life, infants are colonized with multiple bacterial strains whose differences in gene content can have important health consequences. Metagenomics-based approaches have revealed gene content differences between different strains co-colonizing newborns, but less is known about the rate, mechanism, and phenotypic consequences of gene content diversification within strains. Here, focusing on Staphylococcus epidermidis, we whole-genome sequence and phenotype more than 600 isolates from newborns. Within days of birth, infants are co-colonized with a highly personalized repertoire of S. epidermidis strains, which are spread across the newborn body. Comparing the genomes of multiple isolates of each strain, we find very little evidence of adaptive evolution via single-nucleotide polymorphisms. By contrast, we observe gene content differences even between otherwise genetically identical cells, including variation of the clinically important methicillin resistance gene, mecA, suggesting rapid gene gain and loss events at rates higher than point mutations. Mapping the genomic architecture of structural variants by long-read Nanopore sequencing, we find that deleted regions were always flanked by direct repeats, consistent with site-specific recombination. However, we find that even within a single genetic background, recombination occurs at multiple, often non-canonical repeats, leading to the rapid evolution of patient-specific diverse structural variants in the SCCmec island and to differences in antibiotic resistance. Staphylococcus epidermidis is a widespread early colonizer in the neonatal skin and a cause of hospital-acquired infections. Here, using whole-genome sequencing of 632 cultured S. epidermidis isolates derived from premature infants, the authors characterize the spatiotemporally strain-level genomic variability, finding patient-specific colonization signatures and a fast gain and loss of the antibiotic resistance gene mecA via the evolution of genotypically diverse structural variants.", "source_url": "https://www.nature.com/articles/s41467-021-26392-8", "doc_type": 4, "year": 2021, "issue": 1, "volume": 12, "first_page": 1, "last_page": 10, "citation_count": 0, "reference_count": 14, "venue": { "id": 2110000199, "name": "NATURE COMMUNICATIONS", "abbr": "" }, "author": [ { "id": 1001301604, "name": "Manoshi S. Datta" }, { "id": 1001321365, "name": "Idan Yelin" }, { "id": 1003219780, "name": "Ori Hochwald" }, { "id": 1003777919, "name": "Imad Kassis" }, { "id": 1003777920, "name": "Liron Borenstein-Levin" }, { "id": 1003777921, "name": "Amir Kugelman" }, { "id": 1001204603, "name": "Roy Kishony" } ], "field": [ { "id": 2036104400, "name": "2-deoxy-scyllo-inosose synthase" }, { "id": 2041712123, "name": "Tobiano" }, { "id": 2049641606, "name": "HELLS" }, { "id": 2036356743, "name": "Freckle" }, { "id": 2032846693, "name": "Antibiotic resistance" } ], "cite": [ { "name": "GB/T 7714", "text": "Datta Manoshi S., Yelin Idan, Hochwald Ori, et al. Rapid methicillin resistance diversification in Staphylococcus epidermidis colonizing human neonates[J]. NATURE COMMUNICATIONS, 2021, 12(1): 1-10." }, { "name": "MLA", "text": "Datta, Manoshi S., et al. \"Rapid methicillin resistance diversification in Staphylococcus epidermidis colonizing human neonates\" NATURE COMMUNICATIONS., vol. 12, no. 1, 2021, pp. 1-10." }, { "name": "APA", "text": "Datta Manoshi S., Yelin Idan, Hochwald Ori, Kassis Imad, Borenstein-Levin Liron, Kugelman Amir, ... & Kishony Roy (2021). Rapid methicillin resistance diversification in Staphylococcus epidermidis colonizing human neonates. NATURE COMMUNICATIONS, 12(1), 1-10." }, { "name": "BibTeX", "text": "@inproceedings{Acemap3607531,\n title=\"Rapid methicillin resistance diversification in Staphylococcus epidermidis colonizing human neonates\",\n author=\"{Manoshi S. Datta} and Idan {Yelin} and Ori {Hochwald} and Imad {Kassis} and {Liron Borenstein-Levin} and Amir {Kugelman} and Roy {Kishony}\",\n journal=\"NATURE COMMUNICATIONS\",\n volume=\"12\",\n number=\"1\",\n pages=\"1--10\",\n url=\"https://www.acemap.info/paper/3607531\",\n year=\"2021\"\n}" } ] }, { "id": "1866518", "title": "Rapid evolution and host immunity drive the rise and fall of carbapenem resistance during an acute Pseudomonas aeruginosa infection", "abstract": "It is well established that antibiotic treatment selects for resistance, but the dynamics of this process during infections are poorly understood. Here we map the responses of Pseudomonas aeruginosa to treatment in high definition during a lung infection of a single ICU patient. Host immunity and antibiotic therapy with meropenem suppressed P. aeruginosa, but a second wave of infection emerged due to the growth of oprD and wbpM meropenem resistant mutants that evolved in situ. Selection then led to a loss of resistance by decreasing the prevalence of low fitness oprD mutants, increasing the frequency of high fitness mutants lacking the MexAB-OprM efflux pump, and decreasing the copy number of a multidrug resistance plasmid. Ultimately, host immunity suppressed wbpM mutants with high meropenem resistance and fitness. Our study highlights how natural selection and host immunity interact to drive both the rapid rise, and fall, of resistance during infection. Here, following a patient with severe acute Pseudomonas aeruginosa infection, the authors combine comprehensive isolate characterization from lung and gut samples (>100 isolates) and patient clinical data to provide insights into bacterial responses to antibiotic therapy.", "source_url": "https://www.nature.com/articles/s41467-021-22814-9", "doc_type": 4, "year": 2021, "issue": 1, "volume": 12, "first_page": 1, "last_page": 12, "citation_count": 0, "reference_count": 14, "venue": { "id": 2110000199, "name": "NATURE COMMUNICATIONS", "abbr": "" }, "author": [ { "id": 1001758528, "name": "Rachel Wheatley" }, { "id": 1001233138, "name": "Julio Diaz-Caballero" }, { "id": 1001758529, "name": "Natalia Kapel" }, { "id": 1001758530, "name": "Fien H. R. de Winter" }, { "id": 1001758531, "name": "Pramod Jangir" }, { "id": 1001758532, "name": "Angus Quinn" }, { "id": 1001758533, "name": "Ester del Barrio-Tofiño" }, { "id": 1001758534, "name": "Carla López-Causapé" }, { "id": 1001758535, "name": "Jessica Hedge" }, { "id": 1001758536, "name": "Gabriel Torrens" }, { "id": 1001758537, "name": "Thomas Van der Schalk" }, { "id": 1001492182, "name": "Basil Britto Xavier" }, { "id": 1001684371, "name": "Felipe Fernández-Cuenca" }, { "id": 1001758538, "name": "Angel Arenzana" }, { "id": 1001758539, "name": "Claudia Recanatini" }, { "id": 1001758540, "name": "Leen Timbermont" }, { "id": 1001758541, "name": "Frangiscos Sifakis" }, { "id": 1001468194, "name": "Alexey Ruzin" }, { "id": 1001758542, "name": "Omar Ali" }, { "id": 1001492185, "name": "Christine Lammens" }, { "id": 1001492188, "name": "Herman Goossens" }, { "id": 1001758543, "name": "Jan Kluytmans" }, { "id": 1001687930, "name": "Samir Kumar-Singh" }, { "id": 1001684373, "name": "Antonio Oliver" }, { "id": 1001492191, "name": "Surbhi Malhotra-Kumar" }, { "id": 1001683778, "name": "Craig MacLean" } ], "field": [ { "id": 2044760746, "name": "Pseudomonas aeruginosa" }, { "id": 2047474267, "name": "Lividomycin" }, { "id": 2020391647, "name": "Mycobacterium marinum" }, { "id": 2042936613, "name": "Pseudomonas" }, { "id": 2024846241, "name": "Apramycin" }, { "id": 2037369741, "name": "Horizontal resistance" }, { "id": 2049048558, "name": "Pyrenocine" }, { "id": 2017842512, "name": "Tigecycline" }, { "id": 2034944745, "name": "Verdamicin" }, { "id": 2016841655, "name": "Carbapenem" } ], "cite": [ { "name": "GB/T 7714", "text": "Wheatley Rachel, Diaz-Caballero Julio, Kapel Natalia, et al. Rapid evolution and host immunity drive the rise and fall of carbapenem resistance during an acute Pseudomonas aeruginosa infection[J]. NATURE COMMUNICATIONS, 2021, 12(1): 1-12." }, { "name": "MLA", "text": "Wheatley, Rachel, et al. \"Rapid evolution and host immunity drive the rise and fall of carbapenem resistance during an acute Pseudomonas aeruginosa infection\" NATURE COMMUNICATIONS., vol. 12, no. 1, 2021, pp. 1-12." }, { "name": "APA", "text": "Wheatley Rachel, Diaz-Caballero Julio, Kapel Natalia, Winter Fien H. R. de, Jangir Pramod, Quinn Angus, ... & Torrens Gabriel (2021). Rapid evolution and host immunity drive the rise and fall of carbapenem resistance during an acute Pseudomonas aeruginosa infection. NATURE COMMUNICATIONS, 12(1), 1-12." }, { "name": "BibTeX", "text": "@inproceedings{Acemap1866518,\n title=\"Rapid evolution and host immunity drive the rise and fall of carbapenem resistance during an acute Pseudomonas aeruginosa infection\",\n author=\"Rachel {Wheatley} and {Julio Diaz-Caballero} and Natalia {Kapel} and {Fien H. R. de Winter} and Pramod {Jangir} and Angus {Quinn} and {Ester del Barrio-Tofiño} and {Carla López-Causapé} and Jessica {Hedge} and Gabriel {Torrens} and Thomas Van der {Schalk} and Basil Britto {Xavier} and {Felipe Fernández-Cuenca} and Angel {Arenzana} and Claudia {Recanatini} and Leen {Timbermont} and Frangiscos {Sifakis} and Alexey {Ruzin} and Omar {Ali} and Christine {Lammens} and Herman {Goossens} and Jan {Kluytmans} and {Samir Kumar-Singh} and Antonio {Oliver} and {Surbhi Malhotra-Kumar} and Craig {MacLean}\",\n journal=\"NATURE COMMUNICATIONS\",\n volume=\"12\",\n number=\"1\",\n pages=\"1--12\",\n url=\"https://www.acemap.info/paper/1866518\",\n year=\"2021\"\n}" } ] }, { "id": "1738382", "title": "The phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia", "abstract": "Recent studies portend a rising global spread and adaptation of human- or healthcare-associated pathogens. Here, we analyse an international collection of the emerging, multidrug-resistant, opportunistic pathogen Stenotrophomonas maltophilia from 22 countries to infer population structure and clonality at a global level. We show that the S. maltophilia complex is divided into 23 monophyletic lineages, most of which harbour strains of all degrees of human virulence. Lineage Sm6 comprises the highest rate of human-associated strains, linked to key virulence and resistance genes. Transmission analysis identifies potential outbreak events of genetically closely related strains isolated within days or weeks in the same hospitals. Multidrug resistance of the opportunistic pathogen Stenotrophomonas maltophilia is an increasing problem. Here, analyzing strains from 22 countries, the authors show that the S. maltophilia complex is divided into 23 monophyletic lineages and find evidence for intra-hospital transmission.", "source_url": "https://www.nature.com/articles/s41467-020-15123-0", "doc_type": 4, "year": 2020, "issue": 1, "volume": 11, "first_page": 1, "last_page": 12, "citation_count": 1, "reference_count": 5, "venue": { "id": 2110000199, "name": "NATURE COMMUNICATIONS", "abbr": "" }, "author": [ { "id": 1001545793, "name": "Matthias I. Gröschel" }, { "id": 1001559658, "name": "Conor J. Meehan" }, { "id": 1001545792, "name": "Ivan Barilar" }, { "id": 1001559659, "name": "Margo Diricks" }, { "id": 1001559660, "name": "Aitor Gonzaga" }, { "id": 1001206777, "name": "Matthias Steglich" }, { "id": 1001559661, "name": "Oscar Conchillo-Solé" }, { "id": 1001559662, "name": "Isabell-Christin Scherer" }, { "id": 1001559663, "name": "Uwe Mamat" }, { "id": 1001559664, "name": "Christian F. Luz" }, { "id": 1001559665, "name": "Katrien De Bruyne" }, { "id": 1001529301, "name": "Christian Utpatel" }, { "id": 1001559666, "name": "Daniel Yero" }, { "id": 1001559667, "name": "Isidre Gibert" }, { "id": 1001559668, "name": "Xavier Daura" }, { "id": 1001559669, "name": "Stefanie Kampmeier" }, { "id": 1001559670, "name": "Nurdyana Abdul Rahman" }, { "id": 1001559671, "name": "Michael Kresken" }, { "id": 1001559672, "name": "Tjip S. van der Werf" }, { "id": 1001559673, "name": "Ifey Alio" }, { "id": 1001375581, "name": "Wolfgang R. Streit" }, { "id": 1000151640, "name": "Kai Zhou" }, { "id": 1001559674, "name": "Thomas Schwartz" }, { "id": 1001543839, "name": "John W. A. Rossen" }, { "id": 1001559675, "name": "Maha R. Farhat" }, { "id": 1001559676, "name": "Ulrich E. Schaible" }, { "id": 1001206779, "name": "Ulrich Nübel" }, { "id": 1001487676, "name": "Jan Rupp" }, { "id": 1001267518, "name": "Joerg Steinmann" }, { "id": 1001221223, "name": "Stefan Niemann" }, { "id": 1001288640, "name": "Thomas A. Kohl" } ], "field": [ { "id": 2017602054, "name": "Virulence" }, { "id": 2026021454, "name": "Ascochyta fabae" }, { "id": 2003153210, "name": "Mycosphaerella pomi" }, { "id": 2041637869, "name": "Tubakia dryina" }, { "id": 2040988395, "name": "Rhizoctonia oryzae-sativae" }, { "id": 2040655434, "name": "Hansenula subpelliculosa" }, { "id": 2002409167, "name": "Fusarium avenaceum" }, { "id": 2006503831, "name": "Guignardia mangiferae" }, { "id": 2000984322, "name": "Helicobasidium mompa" }, { "id": 2047118759, "name": "Leptosphaerulina trifolii" } ], "cite": [ { "name": "GB/T 7714", "text": "Gröschel Matthias I., Meehan Conor J., Barilar Ivan, et al. The phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia[J]. NATURE COMMUNICATIONS, 2020, 11(1): 1-12." }, { "name": "MLA", "text": "Gröschel, Matthias I., et al. \"The phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia\" NATURE COMMUNICATIONS., vol. 11, no. 1, 2020, pp. 1-12." }, { "name": "APA", "text": "Gröschel Matthias I., Meehan Conor J., Barilar Ivan, Diricks Margo, Gonzaga Aitor, Steglich Matthias, ... & Luz Christian F. (2020). The phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia. NATURE COMMUNICATIONS, 11(1), 1-12." }, { "name": "BibTeX", "text": "@inproceedings{Acemap1738382,\n title=\"The phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia\",\n author=\"{Matthias I. Gröschel} and {Conor J. Meehan} and Ivan {Barilar} and Margo {Diricks} and Aitor {Gonzaga} and Matthias {Steglich} and {Oscar Conchillo-Solé} and {Isabell-Christin Scherer} and Uwe {Mamat} and {Christian F. Luz} and Katrien De {Bruyne} and Christian {Utpatel} and Daniel {Yero} and Isidre {Gibert} and Xavier {Daura} and Stefanie {Kampmeier} and Nurdyana Abdul {Rahman} and Michael {Kresken} and {Tjip S. van der Werf} and Ifey {Alio} and {Wolfgang R. Streit} and Kai {Zhou} and Thomas {Schwartz} and {John W. A. Rossen} and {Maha R. Farhat} and {Ulrich E. Schaible} and {Ulrich Nübel} and Jan {Rupp} and Joerg {Steinmann} and Stefan {Niemann} and {Thomas A. Kohl}\",\n journal=\"NATURE COMMUNICATIONS\",\n volume=\"11\",\n number=\"1\",\n pages=\"1--12\",\n url=\"https://www.acemap.info/paper/1738382\",\n year=\"2020\"\n}" } ] }, { "id": "2323161", "title": "Evolution of drug resistance in an antifungal-naive chronic Candida lusitaniae infection", "abstract": "Management of the limited number of antimicrobials currently available requires the identification of infections that contain drug-resistant isolates and the discovery of factors that promote the evolution of drug resistance. Here, we report a single fungal infection in which we have identified numerous subpopulations that differ in their alleles of a single gene that impacts drug resistance. The diversity at this locus was markedly greater than the reported heterogeneity of alleles conferring antibiotic resistance in bacterial infections. Analysis of genomes from hundreds of Clavispora (Candida) lusitaniae isolates, through individual and pooled isolate sequencing, from a single individual with cystic fibrosis revealed at least 25 nonsynonymous mutations in MRR1, which encodes a transcription factor capable of inducing fluconazole (FLZ) resistance in Candida species. Isolates with high-activity Mrr1 variants were resistant to FLZ due to elevated expression of the MDR1-encoded efflux pump. We found that high Mrr1-regulated Mdr1 activity protected against host and bacterial factors, suggesting drug resistance can be selected for indirectly and perhaps explaining the Mrr1 heterogeneity in this individual who had no prior azole exposure. Regional analysis of C. lusitaniae populations from the upper and lower lobes of the right lung suggested intermingling of subpopulations throughout. Our retrospective characterization of sputum and lung populations by pooled sequencing found that alleles that confer FLZ resistance were a minority in each pool, possibly explaining why they were undetected before unsuccessful FLZ therapy. New susceptibility testing regimes may detect problematical drug-resistant subpopulations in heterogeneous single-species infections.", "source_url": "https://www.pnas.org/content/115/47/12040", "doc_type": 4, "year": 2018, "issue": 47, "volume": 115, "first_page": 12040, "last_page": 12045, "citation_count": 1, "reference_count": 5, "venue": { "id": 2110000201, "name": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA", "abbr": "" }, "author": [ { "id": 1002524042, "name": "Elora G. Demers" }, { "id": 1002524043, "name": "Amy R. Biermann" }, { "id": 1002524044, "name": "Sawyer Masonjones" }, { "id": 1002524045, "name": "Alex W. Crocker" }, { "id": 1001710142, "name": "Alix Ashare" }, { "id": 1001309702, "name": "Jason E. Stajich" }, { "id": 1002416522, "name": "Deborah A. Hogan" } ], "field": [ { "id": 2032846693, "name": "Antibiotic resistance" }, { "id": 2037369741, "name": "Horizontal resistance" }, { "id": 2016841655, "name": "Carbapenem" }, { "id": 2017842512, "name": "Tigecycline" }, { "id": 2010297876, "name": "Faropenem" }, { "id": 2022870316, "name": "Gentamicin" }, { "id": 2043959166, "name": "Ciprofloxacin" }, { "id": 2020391647, "name": "Mycobacterium marinum" } ], "cite": [ { "name": "GB/T 7714", "text": "Demers Elora G., Biermann Amy R., Masonjones Sawyer, et al. Evolution of drug resistance in an antifungal-naive chronic Candida lusitaniae infection[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115(47): 12040-12045." }, { "name": "MLA", "text": "Demers, Elora G., et al. \"Evolution of drug resistance in an antifungal-naive chronic Candida lusitaniae infection\" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA., vol. 115, no. 47, 2018, pp. 12040-12045." }, { "name": "APA", "text": "Demers Elora G., Biermann Amy R., Masonjones Sawyer, Crocker Alex W., Ashare Alix, Stajich Jason E., ... & Hogan Deborah A. (2018). Evolution of drug resistance in an antifungal-naive chronic Candida lusitaniae infection. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(47), 12040-12045." }, { "name": "BibTeX", "text": "@inproceedings{Acemap2323161,\n title=\"Evolution of drug resistance in an antifungal-naive chronic Candida lusitaniae infection\",\n author=\"{Elora G. Demers} and {Amy R. Biermann} and Sawyer {Masonjones} and {Alex W. Crocker} and Alix {Ashare} and {Jason E. Stajich} and {Deborah A. Hogan}\",\n journal=\"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA\",\n volume=\"115\",\n number=\"47\",\n pages=\"12040--12045\",\n url=\"https://www.acemap.info/paper/2323161\",\n year=\"2018\"\n}" } ] }, { "id": "1759613", "title": "Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River", "abstract": "The emergence and widespread distribution of antimicrobial resistant (AMR) bacteria has led to an increasing concern with respect to potential environmental and public health risks. Culture-independent and rapid identification of AMR bacteria in-situ in complex environments is important in understanding the role of viable but non-culturable and antibiotic persistent bacteria and in revealing potential pathogens without waiting for colony formation. In this study, a culture-independent and non-destructive phenotyping approach, so called Raman Deuterium Stable Isotope Probing (Raman-DIP), was developed to identify AMR bacteria in the River Thames. It is demonstrated that Raman-DIP was able to accurately identify resistant and susceptible bacteria within 24 hours. The work shows that, in the River Thames, the majority of the bacteria (76 ± 2%) were metabolically active, whilst AMR bacteria to carbenicillin, kanamycin and both two antibiotics were 35 ± 5%, 28 ± 3%, 25 ± 1% of the total bacterial population respectively. Raman activated cell ejection (RACE) was applied to isolate single AMR bacteria for the first time, linking AMR phenotype (reistance to antibiotics) and genotype (DNA sequence). The sequences of the RACE sorted cells indicate that they were potential human pathogens Aeromonas sp., Stenotrophomonas sp. and an unculturable bacterium. This work demonstrates Raman-DIP and RACE are effective culture-independent approach for rapid identification of AMR bacteria at the single cell level in their natural conditions.", "source_url": "https://www.nature.com/articles/s41598-017-16898-x", "doc_type": 4, "year": 2017, "issue": 1, "volume": 7, "first_page": 1, "last_page": 10, "citation_count": 1, "reference_count": 7, "venue": { "id": 2110000208, "name": "SCIENTIFIC REPORTS", "abbr": "" }, "author": [ { "id": 1001595342, "name": "Yizhi Song" }, { "id": 1000556910, "name": "Li Cui" }, { "id": 1001595343, "name": "José Ángel Siles López" }, { "id": 1000346829, "name": "Jiabao Xu" }, { "id": 1000235070, "name": "Yong-Guan Zhu" }, { "id": 1001595344, "name": "Ian P. Thompson" }, { "id": 1001444840, "name": "Wei E. Huang" } ], "field": [ { "id": 2033870089, "name": "Bacteria" }, { "id": 2024246460, "name": "Thiomonas" }, { "id": 2002515664, "name": "Aminobacter aminovorans" }, { "id": 2032894661, "name": "Pseudomonas aminovorans" }, { "id": 2013333178, "name": "Antibiotic disc" }, { "id": 2025994848, "name": "Bioluminescent bacteria" }, { "id": 2000092662, "name": "Sphingobium francense" }, { "id": 2003763686, "name": "Antimicrobial drug" }, { "id": 2024886425, "name": "Spheroplast" }, { "id": 2003716289, "name": "Lactivicin" } ], "cite": [ { "name": "GB/T 7714", "text": "Song Yizhi, Cui Li, López José Ángel Siles, et al. Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River[J]. SCIENTIFIC REPORTS, 2017, 7(1): 1-10." }, { "name": "MLA", "text": "Song, Yizhi, et al. \"Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River\" SCIENTIFIC REPORTS., vol. 7, no. 1, 2017, pp. 1-10." }, { "name": "APA", "text": "Song Yizhi, Cui Li, López José Ángel Siles, Xu Jiabao, Zhu Yong-Guan, Thompson Ian P., ... & Huang Wei E. (2017). Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River. SCIENTIFIC REPORTS, 7(1), 1-10." }, { "name": "BibTeX", "text": "@inproceedings{Acemap1759613,\n title=\"Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River\",\n author=\"Yizhi {Song} and Li {Cui} and {José Ángel Siles López} and Jiabao {Xu} and {Yong-Guan Zhu} and {Ian P. Thompson} and {Wei E. Huang}\",\n journal=\"SCIENTIFIC REPORTS\",\n volume=\"7\",\n number=\"1\",\n pages=\"1--10\",\n url=\"https://www.acemap.info/paper/1759613\",\n year=\"2017\"\n}" } ] } ]