Melanosome

A melanosome is an organelle found in animal cells and is the site for synthesis, storage and transport of melanin, the most common light-absorbing pigment found in the animal kingdom. Melanosomes are responsible for color and photoprotection in animal cells and tissues. A melanosome is an organelle found in animal cells and is the site for synthesis, storage and transport of melanin, the most common light-absorbing pigment found in the animal kingdom. Melanosomes are responsible for color and photoprotection in animal cells and tissues. Melanosomes are synthesised in the skin in melanocyte cells, as well as the eye in choroidal melanocytes and retinal pigment epithelial (RPE) cells. In lower vertebrates, they are found in melanophores or chromatophores. Melanosomes are relatively large organelles, measuring up to 500 nm in diameter. They are bound by a bilipid membrane and are, in general, rounded, sausage-like, or cigar-like in shape. The shape is constant for a given species and cell type. They have a characteristic ultrastructure on electron microscopy, which varies according to the maturity of the melanosome, and for research purposes a numeric staging system is sometimes used. Melanosomes are dependent for their pigment on certain enzymes, especially tyrosinase, that synthesise the large polymers of melanin within the cell. Before it generates sufficient pigment to be seen on light microscopy it is known as a pre-melanosome. Dysfunction or absence of the melanin-synthesising enzymes (in conditions such as Chédiak–Higashi syndrome) leads to various patterns of albinism. In some melanocytes, the melanosomes remain static within the cell. In others the cell can extend its surface lengthwise as temporary projections known as pseudopodia, which carry melanosomes away from the center of the cell, thereby increasing the cell's effectiveness in absorbing light. The pseudopodial process (aka the tanning process) happens slowly in dermal melanocytes in response to ultraviolet light and to production of new melanosomes and increased donation of melanosomes to adjacent keratinocytes, which are typical skin surface cells. Donation occurs when some keratinocytes engulf the end of the melanocyte pseudopodia, which contain many melanosomes. Cytoplasmic dynein will carry the vesicles containing the melanin to the center of the cell, which causes melanosomes to sequester the keratinocyte's nucleus, providing optimal protection from UV rays. These changes are responsible for tanning of human skin after exposure to UV light or sunlight. In many species of fish, amphibians, crustaceans, and reptiles, melanosomes can be highly mobile within the cell in response to hormonal (or sometimes neural) control, which leads to visible changes in colour that are used for behavioural signaling or photoprotection. Melanosomes found in certain fish species contain pigments that control the color of the fish's scales. Molecular motors, when signaled, will either carry melanosomes containing pigments out to the periphery of the cell, or concentrate them at the center. The motor protein dynein is responsible for concentrating the melanosomes toward the center of the cell, or the 'minus end' of microtubules. Conversely, the protein kinesin is responsible for dispersing the melanosomes to the periphery of the cell, and are plus end directed motors. Because the plus ends of microtubules are oriented towards the periphery, kinesin will carry melanosomes to the periphery. Dispersing melanosomes to the periphery causes the cell to appear darker; concentrating melanosomes towards the center will cause the cell to appear lighter color. This is how a photoprotective system works for the fish on a molecular level.